Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin alphavbeta6 protein-peptide interactions

Wagstaff, J.L. and Vallath, S. and Marshall, J.F. and Williamson, R.A. and Howard, M.J. (2010) Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin alphavbeta6 protein-peptide interactions. Chem Commun (Camb), 46 (40). pp. 7533-7535. ISSN 1359-7345.

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Official URL http://dx.doi.org/10.1039/c0cc01846e

Abstract

We report the first example of peptide-protein heteronuclear two-dimensional (2D) saturation transfer difference nuclear magnetic resonance (STD NMR). This method, resulting in dramatically reduced overlap, was applied to the interaction of the integrin alphavbeta6 with a known peptide ligand and highlights novel contact points between the substrate and target protein.

Item Type:	Article
Additional information:	Wagstaff, Jane L Vallath, Sabari Marshall, John F Williamson, Richard A Howard, Mark J England Chemical communications (Cambridge, England) Chem Commun (Camb). 2010 Oct 28;46(40):7533-5. Epub 2010 Sep 13.
Uncontrolled keywords:	Amino Acid Sequence Antigens, Neoplasm/chemistry/*metabolism Binding Sites Foot-and-Mouth Disease Virus/chemistry/*metabolism Humans Integrins/chemistry/*metabolism Models, Molecular Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular/*methods Peptides/chemistry/*metabolism Protein Binding Receptors, Virus/chemistry/*metabolism
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology T Technology > TP Chemical technology
Divisions:	Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User:	Mark Howard
Date Deposited:	15 Jul 2011 14:42
Last Modified:	18 Nov 2011 11:37
Resource URI:	http://kar.kent.ac.uk/id/eprint/28016 (The current URI for this page, for reference purposes)

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