Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin alphavbeta6 protein-peptide interactions

Wagstaff, Jane L. and Vallath, Sabari and Marshall, John F. and Williamson, Richard A. and Howard, Mark J. (2010) Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin alphavbeta6 protein-peptide interactions. Chemical Communications, 46 (40). pp. 7533-7535. ISSN 1359-7345. (Access to this publication is restricted)

PDF
Restricted to Registered users only
Contact us about this Publication Download (1MB)
[img]
Official URL
http://dx.doi.org/10.1039/c0cc01846e

Abstract

We report the first example of peptide-protein heteronuclear two-dimensional (2D) saturation transfer difference nuclear magnetic resonance (STD NMR). This method, resulting in dramatically reduced overlap, was applied to the interaction of the integrin alphavbeta6 with a known peptide ligand and highlights novel contact points between the substrate and target protein.

Item Type: Article
Additional information: Wagstaff, Jane L Vallath, Sabari Marshall, John F Williamson, Richard A Howard, Mark J England Chemical communications (Cambridge, England) Chem Commun (Camb). 2010 Oct 28;46(40):7533-5. Epub 2010 Sep 13.
Uncontrolled keywords: Amino Acid Sequence Antigens, Neoplasm/chemistry/*metabolism Binding Sites Foot-and-Mouth Disease Virus/chemistry/*metabolism Humans Integrins/chemistry/*metabolism Models, Molecular Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular/*methods Peptides/chemistry/*metabolism Protein Binding Receptors, Virus/chemistry/*metabolism
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
T Technology > TP Chemical technology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Mark Howard
Date Deposited: 15 Jul 2011 14:42
Last Modified: 16 Apr 2014 13:31
Resource URI: http://kar.kent.ac.uk/id/eprint/28016 (The current URI for this page, for reference purposes)
  • Depositors only (login required):

Downloads

Downloads per month over past year