Innocenti, A. and Leewattanapasuk, W. and Manole, G. and Scozzafava, A. and Muhlschlegel, F.A. and Supuran, C.T. (2010) Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase from the pathogenic yeast Candida glabrata with amines and amino acids. Bioorganic & medicinal chemistry letters, 20 (5). pp. 1701-1704. ISSN 1464-3405.
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| Official URL http://dx.doi.org/10.1016/j.bmcl.2010.01.054 |
Abstract
The protein encoded by the NCE103 gene of Candida glabrata, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as CgCA, was investigated for its activation with amines and amino acids. CgCA was weakly activated by amino acids such as l-/d-His, l-Phe, l-DOPA, and l-Trp and by histamine or dopamine (K(A)s of 21.2-37microM) but more effectively activated by d-Phe, d-DOPA, d-Trp as well as serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.1-16.7microM). The best activators were l-/d-Tyr, with activation constants of 7.1-9.5microM. This study may bring a better understanding of the catalytic/activation mechanisms of beta-CAs from pathogenic fungi
| Item Type: | Article |
|---|---|
| Subjects: | Q Science |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group |
| Depositing User: | Sue Davies |
| Date Deposited: | 29 Jun 2011 16:35 |
| Last Modified: | 27 Jun 2012 09:49 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/27484 (The current URI for this page, for reference purposes) |
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