Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase from the pathogenic yeast Candida glabrata with amines and amino acids

Innocenti, Alessio and Leewattanapasuk, Worraanong and Manole, Gheorghe and Scozzafava, Andrea and Mühlschlegel, Fritz A. and Supuran, Claudiu T. (2010) Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase from the pathogenic yeast Candida glabrata with amines and amino acids. Bioorganic & medicinal chemistry letters, 20 (5). pp. 1701-1704. ISSN 1464-3405. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/j.bmcl.2010.01.054

Abstract

The protein encoded by the NCE103 gene of Candida glabrata, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as CgCA, was investigated for its activation with amines and amino acids. CgCA was weakly activated by amino acids such as l-/d-His, l-Phe, l-DOPA, and l-Trp and by histamine or dopamine (K(A)s of 21.2-37microM) but more effectively activated by d-Phe, d-DOPA, d-Trp as well as serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.1-16.7microM). The best activators were l-/d-Tyr, with activation constants of 7.1-9.5microM. This study may bring a better understanding of the catalytic/activation mechanisms of beta-CAs from pathogenic fungi

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 29 Jun 2011 16:35
Last Modified: 09 Apr 2014 15:55
Resource URI: http://kar.kent.ac.uk/id/eprint/27484 (The current URI for this page, for reference purposes)
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