Johnson, M. and Couton, A.T. and Geeves, M.A. and Mulvihill, D.P. (2010) Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS One, 5 (12). e15801. ISSN 1932-6203.
|PDF (Johnson et al)|
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co- expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Dan Mulvihill|
|Date Deposited:||30 Dec 2010 18:10|
|Last Modified:||25 Nov 2011 16:41|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/26186 (The current URI for this page, for reference purposes)|
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