Johnson, M. and Couton, A.T. and Geeves, M.A. and Mulvihill, D.P. (2010) Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS One, 5 (12). e15801. ISSN 1932-6203.
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| Official URL http://dx.plos.org/10.1371/journal.pone.0015801 |
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Abstract
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co- expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | Dan Mulvihill |
| Date Deposited: | 30 Dec 2010 18:10 |
| Last Modified: | 25 Nov 2011 16:41 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/26186 (The current URI for this page, for reference purposes) |
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