Parsons, J.B. and Frank, S. and Bhella, D. and Liang, M. and Prentice, M.B. and Mulvihill, D.P. and Warren, M.J. (2010) Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement. Molecular Cell, 38 . pp. 305-315. ISSN 1097-2765 .
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Compartmentalisation is an important process since it allows the segregation of metabolic activities and, in the era of synthetic biology, represents an important tool by which defined microenvironments can be created for specific metabolic functions. Here we report the synthesis of empty bacterial microcompartments (BMCs) within E. coli cells by the coordinated expression of genes encoding structural shell proteins. A plethora of diverse cellular structures can be generated by changing the expression profile of these genes, including the formation of large axial filaments that interfere with septation. We have shown that proteins can be regiospecially targeted to the empty synthetic organelles and that the targeting element is situated within the N-terminal region of BMC-located proteins. Parallel electron microscopy and live cell imaging approaches confirm the colocalisation of proteins to the microcompartments and provide evidence of filament-associated organelle movement.
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group|
|Depositing User:||Dan Mulvihill|
|Date Deposited:||29 Jun 2011 13:59|
|Last Modified:||23 Nov 2011 09:42|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/24339 (The current URI for this page, for reference purposes)|
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