COUPLING OF D2-DOPAMINE RECEPTORS TO G-PROTEINS IN SOLUBILIZED PREPARATIONS OF BOVINE CAUDATE-NUCLEUS

Chazot, J.A. and Strange, Philip G. (1992) COUPLING OF D2-DOPAMINE RECEPTORS TO G-PROTEINS IN SOLUBILIZED PREPARATIONS OF BOVINE CAUDATE-NUCLEUS. Biochemical Journal, 281 . pp. 369-375. ISSN 0264-6021. (The full text of this publication is not available from this repository)

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Abstract

1. The coupling of D2 dopamine receptors and G-proteins has been examined in cholate-solubilized preparations of bovine caudate nucleus. 2. No receptor-G-protein coupling could be detected in solubilized preparations obtained in 0.3% cholate, but if this preparation is diluted 5-fold, coupling is re-established. 3. The dilution process was examined, and it was shown that the change in ionic strength was an important factor in modulating the observed receptor-G-protein interaction. 4. Ionic strength was shown, however, not to be the primary determinant of receptor-G-protein coupling. This is likely to be the formation, upon dilution of the preparation, of vesicles in which receptor and G-protein reassociate. 5. The formation of vesicles upon dilution was examined by a variety of techniques, including thermal-stability studies, gel filtration, centrifugation and clectron microscopy.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: A. Xie
Date Deposited: 01 Nov 2009 20:08
Last Modified: 17 Apr 2014 08:27
Resource URI: http://kar.kent.ac.uk/id/eprint/23255 (The current URI for this page, for reference purposes)
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