Chazot, J.A. and Strange, P.G. (1992) COUPLING OF D2-DOPAMINE RECEPTORS TO G-PROTEINS IN SOLUBILIZED PREPARATIONS OF BOVINE CAUDATE-NUCLEUS. Biochemical Journal, 281 . pp. 369-375. ISSN 0264-6021.
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Abstract
1. The coupling of D2 dopamine receptors and G-proteins has been examined in cholate-solubilized preparations of bovine caudate nucleus. 2. No receptor-G-protein coupling could be detected in solubilized preparations obtained in 0.3% cholate, but if this preparation is diluted 5-fold, coupling is re-established. 3. The dilution process was examined, and it was shown that the change in ionic strength was an important factor in modulating the observed receptor-G-protein interaction. 4. Ionic strength was shown, however, not to be the primary determinant of receptor-G-protein coupling. This is likely to be the formation, upon dilution of the preparation, of vesicles in which receptor and G-protein reassociate. 5. The formation of vesicles upon dilution was examined by a variety of techniques, including thermal-stability studies, gel filtration, centrifugation and clectron microscopy.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry Q Science > QP Physiology (Living systems) > QP506 Molecular biology |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | A. Xie |
| Date Deposited: | 01 Nov 2009 20:08 |
| Last Modified: | 01 Nov 2009 20:08 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/23255 (The current URI for this page, for reference purposes) |
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