Jarvis, S.M. and Griffith, D.A. (1991) Expression of the rabbit intestinal n2 na+ nucleoside transporter in xenopus-laevis oocytes. Biochemical Journal, 278 . pp. 605-607. ISSN 0264-6021.
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Polyadenylated [poly(A)+] mRNA isolated from rabbit small-intestinal mucosa was injected into Xenopus laevis oocytes, and expression of the N2 Na+/nucleoside co-transporter was assayed by measuring Na+-dependent thymidine uptake. Expression of Na+-dependent thymidine uptake steadily increased after mRNA injection and was on average increased 11-fold by day 6 over background. Na+-dependent thymidine uptake was saturable (apparent K(m) approximately 30-mu-M at 22-degrees-C) and inhibited by uridine and cytidine, but not by guanosine and inosine. These properties of the expressed thymidine transport strongly suggest that the epithelial N2 Na+/nucleoside co-transporter can be expressed in X. laevis oocytes.
|Subjects:||Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||O.O. Odanye|
|Date Deposited:||03 Nov 2009 17:57|
|Last Modified:||03 Nov 2009 17:57|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/22992 (The current URI for this page, for reference purposes)|
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