Purification and properties of p103, a novel 103-kda component of postsynaptic densities

Hayes, Nandini V. L. and Rayner, Denise A. and Baines, Anthony J. (1991) Purification and properties of p103, a novel 103-kda component of postsynaptic densities. Journal of Neurochemistry, 57 (2). pp. 397-405. ISSN 0022-3042. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1111/j.1471-4159.1991.tb03766...

Abstract

A 103-kDa protein present in membrane cytosketal preparations from bovine brain has been identified. We have purified this protein to > 95% homogeneity using gel filtration and ion-exchange chromatography. This protein, p103, is an asymmetric dimer in dilute solution and has two major variants that can be distinguished by isoelectric focussing, pI 5.60 and 5.75. Using subcellular fractionation, it is most enriched in postsynaptic densities. Immunolocalization with anti-p103-specific antibodies reveals that it is confined to the dendrites and perikarya; it is apparently absent from spinal cord axons. It coextracts from brain membrane-skeletal preparations with brain spectrin and actin, but in vitro, it does not interact with them.

Item Type: Article
Uncontrolled keywords: p103; postsynaptic density; neuronal cytoskeleton
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 29 Oct 2009 14:52
Last Modified: 17 Apr 2014 12:56
Resource URI: http://kar.kent.ac.uk/id/eprint/22975 (The current URI for this page, for reference purposes)
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