Development of new procedures for the isolation of phytoplankton DNA from fixed samples

Bertozzini, Elena and Penna, Antonella and Pierboni, Elisa and Bruce, Ian J. and Magnani, Mauro (2005) Development of new procedures for the isolation of phytoplankton DNA from fixed samples. Journal of Applied Phycology, 17 (3). pp. 223-229. ISSN 0921-8971 . (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1007/s10811-005-2130-5

Abstract

Phytoplankton samples collected for routine monitoring programmes have traditionally been preserved with fixatives before subsequent analytical procedures such as microscope-based identification, or simply to permit transport between laboratories. In recent years, to simplify identification and enumeration, the use of DNA or RNA probes coupled with the PCR assay has progressed and now represents a routine procedure for screening cultured and field samples. However, the phytoplankton cells have often still to be treated as fixed samples. The extraction of genomic DNA from fixed cultures of Alexandrium minutum cultures was compared using two new methods based on Magnetisable Solid Phase Support (MSPS) techniques with that using three commercial kits. Genomic DNA recovery and PCR amplification were observed and the results obtained from culture samples were validated using field samples. Among the DNA extraction techniques considered, the MSPS methods provided the best results.

Item Type: Article
Uncontrolled keywords: DNA extraction; fixatives; harmful algal blooms (HABs); magnetisable solid phase support (MSPS); PCR
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Suzanne Duffy
Date Deposited: 11 Sep 2009 14:30
Last Modified: 15 May 2014 08:35
Resource URI: http://kar.kent.ac.uk/id/eprint/22817 (The current URI for this page, for reference purposes)
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