Expression of a class 1 knotted1-like homeobox gene is down-regulated in pea compound leaf primordia.

Hofer, Julie M.I. and Gourlay, Campbell W. and Michael, Anthony and Ellis, T.H. Noel (2001) Expression of a class 1 knotted1-like homeobox gene is down-regulated in pea compound leaf primordia. Plant Molecular Biology, 45 (4). pp. 387-398. ISSN 0167-4412. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1023/A:1010739812836

Abstract

Differences in knotted1-like (knox) gene expression may account for some of the diversity of leaf forms seen in nature. Class 1 knox genes are expressed in the compound leaf primordia of tomato but not in the simple leaf primordia of a range of species examined so far. In order to test the hypothesis that all compound leaves differ from simple leaves in this way, we isolated a class 1 knox cDNA from pea, Pskn1 (Pisum sativum knotted1) and examined its expression pattern. The encoded homeodomain of Pskn1 shares 88% identical residues with KNOTTED1 from maize and an adjacent ELK domain is present. The protein sequence of PSKN1 is 69% identical to TKN2, its nearest related sequence in tomato. Unlike TKn2, Pskn1 was not expressed in newly initiated compound leaves. The expression pattern of Pskn1 resembled those of other class 1 knox genes described in maize and Arabidopsis. Transcripts were detected in the shoot apical meristem and developing vasculature of the vegetative shoot, but expression was not detected in newly initiated and developing compound leaf primordia. The same pattern of expression was observed in the afila mutant, which is characterised by highly ramified compound leaves. Our results suggest that tomato and pea use different developmental processes in the generation of their compound leaves.

Item Type: Article
Uncontrolled keywords: compound leaf; homeobox; pea; plant development
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Campbell Gourlay
Date Deposited: 30 Sep 2009 15:26
Last Modified: 16 Jun 2014 13:14
Resource URI: http://kar.kent.ac.uk/id/eprint/22652 (The current URI for this page, for reference purposes)
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