Glucose-Limited Chemostat Culture of Chinese-Hamster Ovary Cells Producing Recombinant Human Interferon-Gamma

Hayter, Paul M. and Curling, Elizabeth M. and Baines, Anthony J. and Jenkins, Nigel and Salmon, Ian and Strange, Philip G. and Tong, Jeremy M. and Bull, Alan T. (1992) Glucose-Limited Chemostat Culture of Chinese-Hamster Ovary Cells Producing Recombinant Human Interferon-Gamma. Biotechnology and Bioengineering, 39 (3). pp. 327-335. ISSN 0006-3592. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1002/bit.260390311

Abstract

A Chinese hamster ovary (CHO) cell line expressing recombinant human interferon-gamma (IFN-gamma) was grown under glucose limitation in a chemostat at a constant dilution rate of 0.015 h-1 with glucose feed concentrations of 2.75 mM and 4.25 mM. The changes in cell concentration that accompanied changes in the glucose feed concentration indicated that the cells were glucose-limited. The cell yield on glucose remained constant, but there was a decline in residual glucose concentration and a reduced lactate yield from glucose in the latter stages of the culture. The consumption rates for many of the essential amino acids were increased later in the culture. The volumetric rate of interferon-gamma production was maintained throughout the course of this culture, indicating that IFN-gamma expression was stable under these conditions. However, the specific rate of IFN-gamma production was significantly lower at the higher glucose feed concentration. Under glucose limitation, the proportion of fully glycosylated IFN-gamma produced by these cells was less than that produced in the early stages of batch cultures. The proportion of fully glycosylated IFN-gamma increased during transient periods of glucose excess, suggesting that the culture environment influences the glycosylation of IFN-gamma.

Item Type: Article
Uncontrolled keywords: Chinese Hamster Ovary; Interferon-Gamma; Chemostat Culture; Glycosylation
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 08 Sep 2009 07:09
Last Modified: 17 Apr 2014 08:28
Resource URI: http://kar.kent.ac.uk/id/eprint/22611 (The current URI for this page, for reference purposes)
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