Hayter, P.M. and Curling, E.M.A. and Baines, A.J. and Jenkins, N. and Salmon, I. and Strange, P.G. and Tong, J.M. and Bull, A.T. (1992) Glucose-Limited Chemostat Culture of Chinese-Hamster Ovary Cells Producing Recombinant Human Interferon-Gamma. Biotechnology and Bioengineering, 39 (3). pp. 327-335. ISSN 0006-3592.
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A Chinese hamster ovary (CHO) cell line expressing recombinant human interferon-gamma (IFN-gamma) was grown under glucose limitation in a chemostat at a constant dilution rate of 0.015 h-1 with glucose feed concentrations of 2.75 mM and 4.25 mM. The changes in cell concentration that accompanied changes in the glucose feed concentration indicated that the cells were glucose-limited. The cell yield on glucose remained constant, but there was a decline in residual glucose concentration and a reduced lactate yield from glucose in the latter stages of the culture. The consumption rates for many of the essential amino acids were increased later in the culture. The volumetric rate of interferon-gamma production was maintained throughout the course of this culture, indicating that IFN-gamma expression was stable under these conditions. However, the specific rate of IFN-gamma production was significantly lower at the higher glucose feed concentration. Under glucose limitation, the proportion of fully glycosylated IFN-gamma produced by these cells was less than that produced in the early stages of batch cultures. The proportion of fully glycosylated IFN-gamma increased during transient periods of glucose excess, suggesting that the culture environment influences the glycosylation of IFN-gamma.
|Uncontrolled keywords:||Chinese Hamster Ovary; Interferon-Gamma; Chemostat Culture; Glycosylation|
|Subjects:||Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||P. Ogbuji|
|Date Deposited:||08 Sep 2009 07:09|
|Last Modified:||20 May 2011 23:59|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/22611 (The current URI for this page, for reference purposes)|
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