The Volume Changes Associated with the Operation of the Simple Transporter

Hall, A.C. and Jarvis, S.M. (1992) The Volume Changes Associated with the Operation of the Simple Transporter. Biochimica Et Biophysica Acta, 1105 (2). pp. 300-306. ISSN 0006-3002. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/0005-2736(92)90208-4

Abstract

The effects of hydrostatic pressure (0.1-50 MPa) on uridine transport mediated by the 'simple' facilitated nucleoside transporter of guinea-pig and human erythrocytes have been studied in an attempt to identify the volume changes which occur during transport. Pressure inhibited the zero-trans (influx or efflux) mode of uridine transport in guinea-pig cells significantly more (about 2.2-X) than equilibrium exchange. The equilibrium binding of H-3-nitrobenzylthioinosine, a potent specific inhibitor of nucleoside transport, to human red cells and ghosts, was not significantly altered by pressure suggesting that the permeation site was unperturbed. Thus pressure inhibited the transporter primarily by preventing the volume increase associated with the translocation step. Furthermore, the return of the 'empty' transporter was found to be rate-limiting because it required a larger increase in volume than when the transporter was loaded with substrate.

Item Type: Article
Uncontrolled keywords: Nucleoside Transport; Nitrobenzylthioinosine; Hydrostatic Pressure; Erythrocyte; Membrane Transport
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 01 Oct 2009 08:35
Last Modified: 13 Jun 2012 08:34
Resource URI: http://kar.kent.ac.uk/id/eprint/22602 (The current URI for this page, for reference purposes)
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