Glasser, A.L and Eladlouni, C and Keith, G. and Sochacka, E. and Malkiewicz, A. and Santos, M.A.S and Tuite, M.F. and Desgres, J. (1992) Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast. Febs Letters, 314 (3). pp. 381-385. ISSN 0014-5793.
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Abstract
The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.
| Item Type: | Article |
|---|---|
| Uncontrolled keywords: | Trnaleu(U-Asterisk-Aa); Modified Nucleoside; Identification; Mass Spectrometry; Anticodon; Yeast |
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | P. Ogbuji |
| Date Deposited: | 05 Sep 2009 14:42 |
| Last Modified: | 13 Mar 2012 09:41 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/22580 (The current URI for this page, for reference purposes) |
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