Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast

Glasser, Anne-Lise and Eladlouni, Chakib and Keith, Gerard and Sochacka, Elzbieta and Malkiewicz, Andrzej and Santos, Manuel and Tuite, Mick F. and Desgres, Jean (1992) Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast. FEBS Letters, 314 (3). pp. 381-385. ISSN 0014-5793. (The full text of this publication is not available from this repository)

The full text of this publication is not available from this repository. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/0014-5793(92)81510-S

Abstract

The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

Item Type: Article
Uncontrolled keywords: Trnaleu(U-Asterisk-Aa); Modified Nucleoside; Identification; Mass Spectrometry; Anticodon; Yeast
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 05 Sep 2009 14:42
Last Modified: 24 Apr 2014 08:02
Resource URI: http://kar.kent.ac.uk/id/eprint/22580 (The current URI for this page, for reference purposes)
  • Depositors only (login required):