Glasser, A.L and Eladlouni, C and Keith, G. and Sochacka, E. and Malkiewicz, A. and Santos, M.A.S and Tuite, M.F. and Desgres, J. (1992) Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast. Febs Letters, 314 (3). pp. 381-385. ISSN 0014-5793.
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The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.
|Uncontrolled keywords:||Trnaleu(U-Asterisk-Aa); Modified Nucleoside; Identification; Mass Spectrometry; Anticodon; Yeast|
|Subjects:||Q Science > QP Physiology (Living systems) > QP517 Biochemistry|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||P. Ogbuji|
|Date Deposited:||05 Sep 2009 14:42|
|Last Modified:||13 Mar 2012 09:41|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/22580 (The current URI for this page, for reference purposes)|
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