Tuite, M.F. (1992) Strategies for the genetic manipulation of saccharomyces-cerevisiae. Critical Reviews in Biotechnology, 12 (1-2). pp. 157-188. ISSN 0738-8551.
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The budding yeast Saccharomyces cerevisiae is now widely used as a model organism in the study of gene structure, function, and regulation in addition to its more traditional use as a workhorse of the brewing and baking industries. In this article the plethora of methods available for manipulating the genome of S. cerevisiae are reviewed. This will include a discussion of methods for manipulating individual genes and whole chromosomes, and will address both classic genetic and recombinant DNA-based methods. Furthermore, a critical evaluation of the various genetic strategies for genetically manipulating this simple eukaryote will be included, highlighting the requirements of both the new and the more traditional biotechnology industries.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||O.O. Odanye|
|Date Deposited:||27 Jul 2009 22:29|
|Last Modified:||13 Mar 2012 09:41|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/22261 (The current URI for this page, for reference purposes)|
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