Tuite, M.F. (1992) Strategies for the genetic manipulation of saccharomyces-cerevisiae. Critical Reviews in Biotechnology, 12 (1-2). pp. 157-188. ISSN 0738-8551.
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Abstract
The budding yeast Saccharomyces cerevisiae is now widely used as a model organism in the study of gene structure, function, and regulation in addition to its more traditional use as a workhorse of the brewing and baking industries. In this article the plethora of methods available for manipulating the genome of S. cerevisiae are reviewed. This will include a discussion of methods for manipulating individual genes and whole chromosomes, and will address both classic genetic and recombinant DNA-based methods. Furthermore, a critical evaluation of the various genetic strategies for genetically manipulating this simple eukaryote will be included, highlighting the requirements of both the new and the more traditional biotechnology industries.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | O.O. Odanye |
| Date Deposited: | 27 Jul 2009 22:29 |
| Last Modified: | 13 Mar 2012 09:41 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/22261 (The current URI for this page, for reference purposes) |
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