Strategies for the genetic manipulation of saccharomyces-cerevisiae

Tuite, M.F. (1992) Strategies for the genetic manipulation of saccharomyces-cerevisiae. Critical Reviews in Biotechnology, 12 (1-2). pp. 157-188. ISSN 0738-8551. (The full text of this publication is not available from this repository)

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Abstract

The budding yeast Saccharomyces cerevisiae is now widely used as a model organism in the study of gene structure, function, and regulation in addition to its more traditional use as a workhorse of the brewing and baking industries. In this article the plethora of methods available for manipulating the genome of S. cerevisiae are reviewed. This will include a discussion of methods for manipulating individual genes and whole chromosomes, and will address both classic genetic and recombinant DNA-based methods. Furthermore, a critical evaluation of the various genetic strategies for genetically manipulating this simple eukaryote will be included, highlighting the requirements of both the new and the more traditional biotechnology industries.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 27 Jul 2009 22:29
Last Modified: 13 Mar 2012 09:41
Resource URI: http://kar.kent.ac.uk/id/eprint/22261 (The current URI for this page, for reference purposes)
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