Nucleotide-Sequence Of Gene Celm Encoding A New Endoglucanase (Celm) Of Clostridium-Thermocellum And Purification Of The Enzyme

Kobayashi, T. and Romaniec, M.P.M. and Barker, P.J. and Gerngross, U.T. and Demain, A.L. (1993) Nucleotide-Sequence Of Gene Celm Encoding A New Endoglucanase (Celm) Of Clostridium-Thermocellum And Purification Of The Enzyme. Journal of Fermentation and Bioengineering, 76 (4). pp. 251-256. ISSN 0922-338X. (The full text of this publication is not available from this repository)

The full text of this publication is not available from this repository. (Contact us about this Publication)

Abstract

The nucleotide sequence of a new endoglucanase gene (celM) of Clostridium thermocellum was determined. The structural gene contains an open reading frame of 978 bp starting with an ATG codon and terminating in a TGA stop codon. The deduced amino acid sequence of endoglucanase M (CelM) contains 325 amino acids (MW 35,186) and its N-terminal amino acid sequence is identical to that of the purified enzyme. The codon usage of the gene is similar to that of other endoglucanase genes in the same organism. The endoglucanase was purified from Escherichia coli with a 200-fold increase in specific activity. The optimum pH and temperature values of the enzyme are 5.5 to 6.5 and 60-degrees-C respectively for carboxymethylcellulose (CMC) degradation.

Item Type: Article
Subjects: Q Science
R Medicine
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M. Nasiriavanaki
Date Deposited: 26 Jul 2009 15:10
Last Modified: 28 Jul 2009 08:01
Resource URI: http://kar.kent.ac.uk/id/eprint/22165 (The current URI for this page, for reference purposes)
  • Depositors only (login required):