Recombinant enterotoxins as vaccines against escherichia-coli-mediated diarrhea

Aitken, Robert and Hirst, Timothy R. (1993) Recombinant enterotoxins as vaccines against escherichia-coli-mediated diarrhea. In: International Conf on Vaccines for Enteric Diseases, APR 13-15, 1992, Cambridge, England. (The full text of this publication is not available from this repository)

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Abstract

A fusion protein, comprising the B subunit of the heat-labile enterotoxin and a portion of the precursor to the heat-stable enterotoxin of Escherichia coli, has been created by recombinant genetic techniques. It is exported successfully to the bacterial periplasm and assembles into pentamers which retain the ability to bind to GM1 ganglioside. Native toxin epitopes are displayed and the molecule can be easily purified from periplasmic extracts of cells expressing the gene fusion. Although the protein carries the natural sequence of the heat-stable enterotoxin, it is greatly attenuated in toxicity. Systemic immunization of mice or oral administration of the fusion elicits antibody responses against both classes of E. coli enterotoxin.

Item Type: Conference or workshop item (Paper)
Additional information: INTERNATIONAL CONF ON VACCINES FOR ENTERIC DISEASES ROBINSON COLL, CAMBRIDGE, ENGLAND, APR 13-15, 1992 WHO; VACCINE
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: R.F. Xu
Date Deposited: 22 Oct 2009 23:05
Last Modified: 19 Jun 2014 15:24
Resource URI: http://kar.kent.ac.uk/id/eprint/20776 (The current URI for this page, for reference purposes)
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