Structural analysis of tissue inhibitor of metalloproteinases-1 (TIMP-1) by tryptic peptide mapping

Williamson, Richard A. and Smith, Bryan J. and Angal, Sarojani and Murphy, Gillian and Freedman, Robert B. (1993) Structural analysis of tissue inhibitor of metalloproteinases-1 (TIMP-1) by tryptic peptide mapping. Biochimica Et Biophysica Acta, 1164 (1). pp. 8-16. ISSN 0006-3002. (The full text of this publication is not available from this repository)

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Abstract

Tryptic digests of recombinant TIMP-1 have been resolved on reverse-phase HPLC and the major peaks identified by N-terminal sequencing. This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. The peptide map was used to (i), characterize an insoluble 'core' peptide seen on digestion of TIMP-1 in non-reducing conditions; (ii). confirm the structure of DELTA127-184TIMP-1, a recently described truncated form of the TIMP-1 molecule; (iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale.

Item Type: Article
Uncontrolled keywords: tissue inhibitor; proteinase inhibitor; metalloproteinase; peptide mapping; proteolysis
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 13 Jul 2009 18:16
Last Modified: 02 Jun 2014 13:54
Resource URI: http://kar.kent.ac.uk/id/eprint/20742 (The current URI for this page, for reference purposes)
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