Structural analysis of tissue inhibitor of metalloproteinases-1 (TIMP-1) by tryptic peptide mapping

Williamson, Richard A., Smith, Bryan J., Angal, Sarojani, Murphy, Gillian, Freedman, Robert B. (1993) Structural analysis of tissue inhibitor of metalloproteinases-1 (TIMP-1) by tryptic peptide mapping. Biochimica Et Biophysica Acta, 1164 (1). pp. 8-16. ISSN 0006-3002. (doi:10.1016/0167-4838(93)90105-z) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:20742)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: https://doi.org/10.1016/0167-4838(93)90105-z

Abstract

Tryptic digests of recombinant TIMP-1 have been resolved on reverse-phase HPLC and the major peaks identified by N-terminal sequencing. This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. The peptide map was used to (i), characterize an insoluble 'core' peptide seen on digestion of TIMP-1 in non-reducing conditions; (ii). confirm the structure of DELTA127-184TIMP-1, a recently described truncated form of the TIMP-1 molecule; (iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale.

Item Type:	Article
DOI/Identification number:	10.1016/0167-4838(93)90105-z
Uncontrolled keywords:	tissue inhibitor; proteinase inhibitor; metalloproteinase; peptide mapping; proteolysis
Subjects:	Q Science > QP Physiology (Living systems) > QP517 Biochemistry Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	O.O. Odanye
Date Deposited:	13 Jul 2009 18:16 UTC
Last Modified:	09 Mar 2023 11:31 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/20742 (The current URI for this page, for reference purposes)

University of Kent Author Information

Williamson, Richard A..

Creator's ORCID:
CReDIT Contributor Roles:

Freedman, Robert B..

Creator's ORCID:
CReDIT Contributor Roles:

Depositors only (login required):

Altmetric

Download Statistics

Total unique views for this document in KAR since July 2020. For more details click on the image.