Williamson, R.A. and Smith, B.J. and Angal, S. and Murphy, G. and Freedman, R.B. (1993) Structural analysis of tissue inhibitor of metalloproteinases-1 (TIMP-1) by tryptic peptide mapping. Biochimica Et Biophysica Acta, 1164 (1). pp. 8-16. ISSN 0006-3002.
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Tryptic digests of recombinant TIMP-1 have been resolved on reverse-phase HPLC and the major peaks identified by N-terminal sequencing. This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. The peptide map was used to (i), characterize an insoluble 'core' peptide seen on digestion of TIMP-1 in non-reducing conditions; (ii). confirm the structure of DELTA127-184TIMP-1, a recently described truncated form of the TIMP-1 molecule; (iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale.
|Uncontrolled keywords:||tissue inhibitor; proteinase inhibitor; metalloproteinase; peptide mapping; proteolysis|
|Subjects:||Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||O.O. Odanye|
|Date Deposited:||13 Jul 2009 18:16|
|Last Modified:||30 May 2012 10:53|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/20742 (The current URI for this page, for reference purposes)|
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