Teare, J.P and Carmichael, A.J. and Burnett, F.R and Rake, M.O (1993) Detection of antibodies to acetaldehyde-albumin conjugates in alcoholic liver disease. Alcohol and Alcoholism, 28 (1). pp. 11-16. ISSN 0735-0414.
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The principal metabolite of ethanol, acetaldehyde, conjugates with various proteins that form antibody-inducing neo-antigens. We have analysed sera from patients with biopsy-proven alcoholic liver disease (hepatitis = 10, cirrhosis = 11, steatosis = 3) and controls = 19 (normal teetotallers and 6 non-alcoholic liver disease). Sera were examined with an enzyme-linked immunoabsorbent assay (ELISA) for antibodies binding preferentially to an acetaldehyde-albumin conjugate. Reactive sera from alcohol misusers were then purified using an amino-hexyl Sepharose affinity column. Antibodies binding to the acetaldehyde-albumin epitopes were significantly raised (P < 0.005) in all groups of alcohol misusers, and were present in greatest titre in those with alcoholic hepatitis. These antibodies were successfully purified using the gel affinity column. We conclude that alcohol misusers have significant titres of antibodies reacting to the acetaldadehyde-albumin complex. The role of these antibodies remains unclear, but may be related to the initiation of an inflammatory response and tissue damage following ethanol consumption.
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences
Faculties > Science Technology and Medical Studies > Kent Institute of Medicine and Health Sciences (KIMHS)
|Depositing User:||O.O. Odanye|
|Date Deposited:||20 Jul 2009 20:33|
|Last Modified:||20 Apr 2012 14:51|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/20712 (The current URI for this page, for reference purposes)|
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