Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride

Byrne, Lee J. and Cox, Brian S. and Cole, Diana J. and Ridout, Martin S. and Morgan, Byron J. T. and Tuite, Mick F. (2007) Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride. Proceedings of the National Academy of Sciences of the United States of America, 104 (28). pp. 11688-11693. ISSN 0027-8424. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1073/pnas.0701392104

Abstract

Guanidine hydrochloride (Gdn center dot HCl) blocks the propagation of yeast prions by inhibiting Hsp104, a molecular chaperone that is absolutely required for yeast prion propagation. We had previously proposed that ongoing cell division is required for Gdn center dot HCl-induced loss of the [PSI+] prion. Subsequently, Wu et al. [Wu Y, Greene LE, Masison DC, Eisenberg E (2005) Proc Nat] Acad Sci USA 102:1278912794] claimed to show that Gdn center dot HCl can eliminate the [PSI+] prion from alpha-factor-arrested cells leading them to propose that in Gdn center dot HCl center dot treated cells the prion aggregates are degraded by an Hsp104-independent mechanism. Here we demonstrate that the results of Wu et al can be explained by an unusually high rate of alpha-factor-induced cell death in the [PSI+] strain (780-1D) used in their studies. What appeared to be no growth in their experiments was actually no increase in total cell number in a dividing culture through a counterbalancing level of cell death. Using media-exchange experiments, we provide further support for our original proposal that elimination of the [PSI+] prion by Gdn center dot HCl requires ongoing cell division and that prions are not destroyed during or after the evident curing phase.

Item Type: Article
Uncontrolled keywords: propagons; Hsp104; alpha-factor
Subjects: Q Science > QA Mathematics (inc Computing science)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Mathematics Statistics and Actuarial Science
Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Stephen Holland
Date Deposited: 19 Dec 2007 19:25
Last Modified: 14 May 2014 15:26
Resource URI: http://kar.kent.ac.uk/id/eprint/2054 (The current URI for this page, for reference purposes)
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