Effect of Lipid Supplements on the Production and Glycosylation of Recombiant Interferon-Gamma Expressed in CHO Cells

Jenkins, N. and Castro, P. and Menon, S. and Ison, A.P. and Bull, A.T. (1994) Effect of Lipid Supplements on the Production and Glycosylation of Recombiant Interferon-Gamma Expressed in CHO Cells. In: Cell Culture Engineering IV Conference, San Diego, Ca. (The full text of this publication is not available from this repository)

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Abstract

The effects of lipids on the glycosylation of recombinant human interferon-gamma expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-grycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-gamma deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-gamma glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.

Item Type: Conference or workshop item (Other)
Uncontrolled keywords: GLYCOSYLATION; RECOMBINANT; INTERFERON; CHO CELLS; LIPIDS
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 25 Jun 2009 11:38
Last Modified: 27 Jun 2012 10:28
Resource URI: http://kar.kent.ac.uk/id/eprint/20272 (The current URI for this page, for reference purposes)
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