Mistranslation of Human Phosphoglycerate Kinase in Y east in the Presence of Paromomycin

Grant, Chris M. and Tuite, Mick F. (1994) Mistranslation of Human Phosphoglycerate Kinase in Y east in the Presence of Paromomycin. Current Genetics, 26 (2). pp. 95-99. ISSN 0172-8083. (The full text of this publication is not available from this repository)

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Abstract

Missense errors in the translation of mRNAs in Saccharomyces cerevisiae were screened by looking for charge heterogeneity of proteins on two-dimensional gels resulting from the substitution of charged and neutral amino acids. No such mistranslation was detected in wildtype yeast strains grown in the presence of the translational error-inducing antibiotic paromomycin. However, paromomycin-induced mistranslation of a heterologous, mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen. We suggest that the combination of error-prone translation of a heterologous mRNA, and growth in the presence of paromomycin, leads to an accumulation of mistranslated proteins that can be detected by two-dimensional gel electrophoresis.

Item Type: Article
Uncontrolled keywords: TRANSLATIONAL FIDELITY; PAROMOMYCIN; STUTTERING; SACCHAROMYCES CEREVISIAE
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 26 Jun 2009 15:20
Last Modified: 07 Jul 2014 14:15
Resource URI: http://kar.kent.ac.uk/id/eprint/20222 (The current URI for this page, for reference purposes)
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