Grant, C.M. and Tuite, M.F. (1994) Mistranslation of Human Phosphoglycerate Kinase in Y east in the Presence of Paromomycin. Current Genetics, 26 (2). pp. 95-99. ISSN 0172-8083.
|The full text of this publication is not available from this repository. (Contact us about this Publication)|
Missense errors in the translation of mRNAs in Saccharomyces cerevisiae were screened by looking for charge heterogeneity of proteins on two-dimensional gels resulting from the substitution of charged and neutral amino acids. No such mistranslation was detected in wildtype yeast strains grown in the presence of the translational error-inducing antibiotic paromomycin. However, paromomycin-induced mistranslation of a heterologous, mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen. We suggest that the combination of error-prone translation of a heterologous mRNA, and growth in the presence of paromomycin, leads to an accumulation of mistranslated proteins that can be detected by two-dimensional gel electrophoresis.
|Uncontrolled keywords:||TRANSLATIONAL FIDELITY; PAROMOMYCIN; STUTTERING; SACCHAROMYCES CEREVISIAE|
|Subjects:||Q Science > QH Natural history > QH426 Genetics|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||P. Ogbuji|
|Date Deposited:||26 Jun 2009 15:20|
|Last Modified:||12 Jun 2012 09:38|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/20222 (The current URI for this page, for reference purposes)|
- Depositors only (login required):