Teneberg, S. and Hirst, T.R. and Angstrom, J. and Karlsson, K.A. (1994) Comparison of the Glycolipid-Binding Specificities of Cholera-Toxin and Porcin Escherichia-Coli Heart-Labile Enterotoxin - Identification of a Receptor-Active Non-Ganglioside Glycolipid for the Heart-Labile toxin in Infant Rabit Small- Intenstin. Glycoconjugate Journal, 11 (6). pp. 533-540. ISSN 0282-0080.
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Abstract
The binding specificities of cholera toxin and Escherichia coli heat-labile enterotoxin were investigated by binding of I-125-labelled toxins to reference glycosphingolipids separated on thin-layer chromatograms and coated in microtitre wells. The binding of cholera toxin was restricted to the GM1 ganglioside. The heat-labile toxin showed the highest affinity for GM1 but also bound, though less strongly, to the GM2, GD2 and GD1b gangliosides and to the non-acid glycosphingolipids gangliotetraosylceramide and lactoneotetraosylceramide. The infant rabbit small intestine, a model system for diarrhoea induced by the toxins, was shown to contain two receptor-active glycosphingolipids for the heat-labile toxin, GM1 ganglioside and lactoneotetraosylceramide, whereas only the GM1 ganglioside was receptor-active for cholera toxin. Preliminary evidence was obtained, indicating that epithelial cells of human small intestine also contain lactoneotetraosylceramide and similar sequences. By computer-based molecular modelling, lactoneotetraosylceramide was docked into the active site of the heat-labile toxin, using the known crystal structure of the toxin in complex with lactose. Interactions which may explain the relatively high toxin affinity for this receptor were found.
| Item Type: | Article |
|---|---|
| Uncontrolled keywords: | GLYCOLIPID RECEPTOR; CHOLERA TOXIN; PORCINE ESCHERICHIA COLI HEAT-LABILE ENTEROTOXIN; INFANT RABBIT INTESTINE |
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | P. Ogbuji |
| Date Deposited: | 18 Jun 2009 08:50 |
| Last Modified: | 24 Jul 2012 14:46 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/20070 (The current URI for this page, for reference purposes) |
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