Poole, R.K and Salmon, I. and Chance, B. (1994) The high-spin cytochrome o’ component of the cytochrome bo-type quinol oxidase in membranes from Escherichia coli: formation of the primary oxygenated species at low temperatures is characterized by a slow 'on' rate and low dissociation constant. Microbiology-Uk, 140 . pp. 1027-1034. ISSN 1350-0872.
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Cytochromes b and o in membrane vesicles from aerobically grown Escherichia coil were readily reduced by succinate; one cytochrome, which we propose should be called cytochrome o', reacted with CO in the Fe(II) state to give a photodissociable CO adduct. The photodissociation spectrum (photolysed minus pre-photolysis) at sub-zero temperatures had a relatively high gamma/alpha absorbance ratio, indicating a high-spin haem, which, in the reduced state, probably contributes little to the sharp a absorbance of the oxidase complex in membranes. Reaction with oxygen of the unliganded high-spin haem between -132 degrees C and -95 degrees C following photolytic activation gave a product that is identified as the oxygenated form, being spectrally similar to, but not identical with, the CO adduct. In membranes, the forward velocity constant at -95 degrees C was 61 M(-1) s(-1), and the dissociation constant was 1.6 x 10(-5) M O-2, as it is in intact cells. These data clearly distinguish the oxygen-trapping strategy of the cytochrome o' in this oxidase from that of cytochrome a(3) and also suggest that the presence of the soluble flavohaemoglobin (Hmp) in intact cells is without effect on such measurements of the primary oxygen reaction. In view of recent findings that this oxidase complex contains predominantly one mole of haem O and one of haem B. a revised nomenclature for the oxidase complex is proposed, namely, cytochrome bo'.
|Additional information:||Author for correspondence: Robert K. Poole. Tel: +1 607 255 2423. Fax: +1 607 255 3904.|
|Uncontrolled keywords:||Escberichia coli, cytochrome bo, cytochrome o, electron transfer, quinol oxidase, oxygen reduction|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||O.O. Odanye|
|Date Deposited:||16 Jun 2009 01:45|
|Last Modified:||16 Jun 2009 01:45|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/19952 (The current URI for this page, for reference purposes)|
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