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Mammalian-cell damage in a novel membrane bioreactor

Millward, H.R., Bellhouse, B.J., Nicholson, Andrea M., Beeton, S., Jenkins, Nigel, Knowles, Christopher J. (1994) Mammalian-cell damage in a novel membrane bioreactor. Biotechnology and Bioengineering, 43 (9). pp. 899-906. ISSN 0006-3592. (doi:10.1002/bit.260430909) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19911)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1002/bit.260430909

Abstract

The experimental study has assessed a novel membrane bioreactor for mammalian cell culture. In the absence of a gas phase, the key features of cell damage associated with laminar and turbulent flow have been identified. The bioreactor employs a dimpled membrane in order to enhance transverse mixing in a narrow channel, but a fall in viable cell density has been observed at Reynolds numbers above Re = 83. In the laminar flow regime wall shear is the critical mechanism and an accurate calculation of shear rate in a complex channel has been achieved using the Reynolds analogy. Flow generating a wall shear rate in excess of 3000 s(-1) has been shown to cause damage. Power dissipation measurements have been used to distinguish between laminar and turbulent flow and also to predict Kolmogorov eddy lengths. An additional turbulent bulk stress damage mechanism at higher Reynolds numbers (Re > 250) results in a very rapid fall in viable cell density.

Item Type: Article
DOI/Identification number: 10.1002/bit.260430909
Uncontrolled keywords: membrane bioreactor; mammalian cell damage; critical shear rate; power dissipation
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: O.O. Odanye
Date Deposited: 06 Jul 2009 19:55 UTC
Last Modified: 16 Nov 2021 09:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19911 (The current URI for this page, for reference purposes)

University of Kent Author Information

Knowles, Christopher J..

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