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An artificial wax substrate for the in-vitro study of phylloplane microorganisms

McCormack, P.J, Bailey, Mark A., Jeffries, Peter (1994) An artificial wax substrate for the in-vitro study of phylloplane microorganisms. Journal of Microbiological Methods, 19 (1). pp. 19-28. ISSN 0167-7012. (doi:10.1016/0167-7012(94)90022-1) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19885)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
https://doi.org/10.1016/0167-7012(94)90022-1

Abstract

An artificial substrate consisting of a wax-based medium amended with nutrients has been developed for the in vitro study of microbial interactions on the phylloplane. Inoculation of bacteria and yeasts onto the surface demonstrated that these micro-organisms remain viable for up to 13 days (e.g. Aureobasidium pullulans) and proliferate on the surface under conditions of low humidity. The growth rate was slower (doubling time 1.2 days) than on nutrient agar or in nutrient broth. The artificial leaf wax substrate supported a lower bacterial population (10(6)-10(7) c.f.u. plate(-1)) than conventional nutrient substrates. Inoculation with Aureobasidium pullulans in high (10(9) c.f.u. plate(-1)) or low (10(4) c.f.u. plate(-1)) numbers indicated that the surface could support between 16 x 10(4)-16 x 10(5) c.f.u. per cm(2). In the absence of incorporated nutrients, yeast numbers were found not to increase. Spore germination and mycelial elongation of filamentous fungi also occurred on the artificial leaf wax substrate but rates were reduced in comparison with those on nutrient agar. Inoculation of the artificial leaf substrate with a mixed population of a soil yeast and a phyllosphere-specific yeast resulted in competition between species such that the phyllosphere yeast became dominant.

Item Type: Article
DOI/Identification number: 10.1016/0167-7012(94)90022-1
Uncontrolled keywords: aureobasidium pullulans; leaf model; phylloplane; yeast
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: O.O. Odanye
Date Deposited: 07 Jul 2009 18:51 UTC
Last Modified: 09 Mar 2023 11:31 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19885 (The current URI for this page, for reference purposes)

University of Kent Author Information

Jeffries, Peter.

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