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Efficient extracellular production of hybrid escherichia-coli heat-labile enterotoxin-b subunits in a marine vibrio

Marcello, Alessandro, Loregian, Arianna, Palu, Giorgio, Hirst, Timothy R. (1994) Efficient extracellular production of hybrid escherichia-coli heat-labile enterotoxin-b subunits in a marine vibrio. FEMS Microbiology Letters, 117 (1). pp. 47-51. ISSN 0378-1097. (doi:10.1111/j.1574-6968.1994.tb06741.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19878)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1111/j.1574-6968.1994.tb06741...

Abstract

Escherichia coil heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically engineered that correspond to a well-characterized neutralising epitope of glycoprotein D from herpes simplex virus (EtxB-gD) and to the C-terminal nine amino acids from the 38 kDa subunit of HSV-encoded ribonucleotide reductase (EtxB-R2). Here we describe the extracellular secretion of the two hybrid EtxBs from a marine Vibrio harbouring a broad-host range inducible expression vector containing the hybrid genes. Large amounts of intact fusion proteins (15-20 mg per liter of culture) were secreted into the medium upon induction. These hybrid proteins maintained the receptor-binding activity of the native toxin as well as being cross-reactive with anti-EtxB and anti-heterologous peptide monoclonal antibodies.

Item Type: Article
DOI/Identification number: 10.1111/j.1574-6968.1994.tb06741.x
Uncontrolled keywords: escherichia-coli heat-labile enterotoxin; vibrio sp 60; heterologous expression, secretion
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: O.O. Odanye
Date Deposited: 07 Jul 2009 20:21 UTC
Last Modified: 16 Nov 2021 09:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19878 (The current URI for this page, for reference purposes)

University of Kent Author Information

Hirst, Timothy R..

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