Heterogeneity within Populations of Recombination Chinese-Haster Ovary Cells Epressing Human Interferon Gamma

Coppen, S.R and Newsam, Ray J. and Bull, Alan T. and Baines, Anthony J. (1995) Heterogeneity within Populations of Recombination Chinese-Haster Ovary Cells Epressing Human Interferon Gamma. Biotechnology and Bioengineering, 46 (2). pp. 147-158. ISSN 0006-3592. (The full text of this publication is not available from this repository)

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Abstract

The Chinese hamster ovary (CHO) cell line has great commercial importance in the production of recombinant human proteins, especially those for therapeutic use. Much attention has been paid to CHO cell population physiology in order to define factors affecting product fidelity and yield. Such studies have revealed that recombinant proteins, including human interferon-gamma (IFN-gamma), can be heterogeneous both in glycosylation and in proteolytic processing. The type of heterogeneity observed depends on the growth physiology of the cell population, although the relationship between them is complex. In this article we report results of a cytological study of the CH0320 line which expresses recombinant human IFN-gamma. When grown in suspension culture,this cell line exhibited three types of heterogeneity: (1) heterogeneity of the production of IFN-gamma within the cell population, (2) heterogeneity of the number of nuclei and mitotic spindles in dividing cells, and (3) heterogeneity of cellular environment. The last of these arises from cell aggregates which form in suspension culture: Some cells are exposed to the culture medium; others are fully enclosed within the mass with little or no direct access to the medium. Thus, live cells producing IFN-gamma are heterogeneous in their environment, with variable access to O-2 and nutrients. Within the aggregates, it appears that live cells proliferate on a dead cell mass. The layer of live cells can be several cells deep. Specific cell-cell attachments are observed between the living cells in these aggregates. Two proteins, known to be required for the formation of certain types of intercellular junctions, spectrin and vinculin, have been localized to the regions of cell-cell contact. The aggregation of the cells appears to be an active process requiring protein synthesis (C) 1995 John Wiley and Sons, Inc.

Item Type: Article
Uncontrolled keywords: CHO CELL; CELL AGGREGATION; RECOMBINANT HUMAN INTERFERON-GAMMA; MAMMALIAN CELL CULTURE; CELL CULTURE
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 05 Jun 2009 16:37
Last Modified: 23 Jun 2014 13:29
Resource URI: http://kar.kent.ac.uk/id/eprint/19772 (The current URI for this page, for reference purposes)
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