Immunolocalization of Nucleoside Transports in Human Placental Trophoblasi and Endothelial-Cells - Evidence for Multiple Transporter Isoforms

Barros, L.F. and Yudilevich, D.L. and Jarvis, S.M. and Beaumont, N. and Young, J.D. and Baldwin, S.A. (1995) Immunolocalization of Nucleoside Transports in Human Placental Trophoblasi and Endothelial-Cells - Evidence for Multiple Transporter Isoforms. Pflugers Archiv-European Journal of Physiology, 429 (3). pp. 394-399. ISSN 0031-6768. (The full text of this publication is not available from this repository)

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Abstract

Polyclonal antibodies raised against the human erythrocyte nucleoside transporter were used to investigate the distribution of the nucleoside transporters in the placenta. Immunoblots of brush-border membranes isolated from the human syncytiotrophoblast revealed a cross-reactive species that co-migrated with the erythrocyte nucleoside transporter as a broad band of apparent M(r) 55,000. In contrast, no labelling was detected in basal membranes containing a similar number of equilibrative nucleoside transporters as assessed by nitrobenzylthioinosine (NBMPR)-binding. The absence of cross-reactive epitopes in basal membranes and their presence in brush-border membranes was confirmed by confocal immunofluorescence microscopy. The results suggest that at least two isoforms of the NBMPR-sensitive nucleoside transporter are present in the human placenta. The lumenal surfaces of fetal capillaries, small placental vessels and umbilical vein ware also strongly labelled by the antibody, a finding that suggests that the high fetal-placental adenosine uptake previously reported is due to endothelial transporters.

Item Type: Article
Uncontrolled keywords: NUCLEOSIDE TRANSPORT; NITROBENZYLTHIOINOSINE (NBMPR); MICROVILLOUS AND BASAL MEMBRANE VESICLES; PLACENTA (HUMAN)
Subjects: B Philosophy. Psychology. Religion > B Philosophy (General)
Divisions: Faculties > Social Sciences > School of Psychology
Depositing User: P. Ogbuji
Date Deposited: 08 Jun 2009 16:23
Last Modified: 17 Jul 2012 11:02
Resource URI: http://kar.kent.ac.uk/id/eprint/19688 (The current URI for this page, for reference purposes)
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