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Quantitation and aiammunolocalization of Glucose Transporters in the Human Placenta

Barros, L.F., Yudilevich, D.L., Jarvis, Simon M., Beaumont, N., Baldwin, S.A. (1995) Quantitation and aiammunolocalization of Glucose Transporters in the Human Placenta. Placenta, 16 (7). pp. 623-633. ISSN 0143-4004. (doi:10.1016/0143-4004(95)90031-4) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19687)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1016/0143-4004(95)90031-4

Abstract

The subcellular distributions of the mammalian passive glucose transporter isoforms GLUT1, GLUT3 and GLUT4,* in the human placenta, were investigated using isoform-specific anti-peptide antibodies. On western blots of both basal and brush-bolder plasma membranes isolated from the syncytiotrophoblast, antibodies specific for GLUT1 labelled a broad band (apparent Mr 55 000) that co-migrated with the human erythrocyte GLUT1 glucose transporter. In contrast, no labelling was detectable when blots mere probed with antibodies specific for the GLUT3 or GLUT4 isoforms. Densitometric analysis of blots showed that GLUT1 accounts for approximately 90 and 65 per cent of the D-glucose-sensitive cytochalasin B binding sites present in brush-border and basal membranes, respectively. Confocal immunofluorescence microscopy of fixed placental tissue showed that GLUT1 is abundant at both maternal- and fetal-facing surfaces of the syncytiotrophoblast whereas it was undetectable at the fetal capillary endothelium. In parallel experiments, no staining by antibodies against either the GLUT3 or the GLUT4 isoforms mns detected in placental tissue. These results indicate that GLUT1 is the major isoform responsible for glucose transfer from mother to fetus. The absence of GLUT4 is consistent with the lack of insulin-sensitive glucose transport across the placenta.

Item Type: Article
DOI/Identification number: 10.1016/0143-4004(95)90031-4
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: P. Ogbuji
Date Deposited: 08 Jun 2009 16:31 UTC
Last Modified: 16 Nov 2021 09:57 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19687 (The current URI for this page, for reference purposes)

University of Kent Author Information

Jarvis, Simon M..

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