Jolley, C. and Burnet, F.R. and Blower, P.J. (1996) Improved synthesis and characterisation of a hydrazide derivative of diethylenetriaminepentaacetic acid for site-specific labelling of monoclonal antibodies with In-111. Applied Radiation and Isotopes, 47 (7). pp. 623-626. ISSN 0969-8043.
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The monohydrazide derivative of diethylenetriaminepentaacetic acid, (HOOCCH2)(2)NCH2CH2N(CH2COOH)CH2CH2N(CH2COOH)(CH2CO.NHNH2), is a bifunctional chelator designed for attaching the radiometal In-111 selectively to the carbohydrate side chains of pre-oxidised monoclonal antibodies. A simple synthesis of this chelator (from diethylenetriaminepentaacetic acid cyclic anhydride and hydrazine), and its purification and chemical characterisation, are described. Rabbit IgG was oxidised with periodate, and the aldehyde groups thus generated were reacted with the linker forming a conjugate that was readily labelled in high yield and purity with In-111.
|Subjects:||Q Science > QD Chemistry|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||R.F. Xu|
|Date Deposited:||27 May 2009 15:02|
|Last Modified:||27 May 2009 15:02|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/19268 (The current URI for this page, for reference purposes)|
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