Survival Of Inoculated Bacillus-Cereus Spores And Vegetative Cells In Non-Planted And Rhizosphere Soil

Young, C.S. and Lethbridge, Gordon and Shaw, L.J. and Burns, Richard G. (1995) Survival Of Inoculated Bacillus-Cereus Spores And Vegetative Cells In Non-Planted And Rhizosphere Soil. Soil Biology and Biochemistry, 27 (8). pp. 1017-1026. ISSN 0038-0717. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/0038-0717(95)00030-I

Abstract

The survival of vegetative cells and spores of an antibiotic-resistant Bacillus cereus (B11) inoculant was similar in wheat rhizosphere soil and non-planted soil over a period of 21 d. Reliable ways of producing either spore or vegetative single cell inocula were developed. When applied to give densities ranging from 1 x 10(2) t o 1 x 10(7) g(-1) soil, B11 spores survived for at least 21 d at levels similar to those introduced but numbers of vegetative cells decreased by 1-2 orders of magnitude within the first 48 h and the extent of decrease was proportionately greater at low inoculum density. There was no significant difference after 21 d between total counts (vegetative cells + spores) or spore counts in either the rhizosphere or non-planted soil, regardless of whether the inoculant was composed of vegetative or spore cells. There was no difference between the changing spore-to-vegetative cell ratio within total cell counts between rhizosphere and non-planted soil. Inoculating soil with a mixture of Bacillus B11 (2 x 10(7) g(-1)), Flavobacterium P25 (3 x 10(8) g(-1)) and Arthrobacter A109 (2 x 10(8) g(-1)) increased the survival of P25 and A109 but decreased the survival of B11 in both non-planted and rhizosphere soil compared with each bacterium inoculated alone. For example, at day 21 in rhizosphere soil there were 1 x 10(6) g (-1) A109 (single inoculant) and 1 x 10(7) g(-1) A109 (mixed inoculum) and in non-planted soil 1 x 10(5) g(-1) (single) and 1 x 10(7) g(-1) (mixed). Thus, neither the survival nor the physiological state of Bacillus B11 was affected by the presence of developing wheat roots but survival was negatively affected by the presence of other inoculants.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 30 Oct 2009 17:26
Last Modified: 06 May 2014 13:39
Resource URI: http://kar.kent.ac.uk/id/eprint/19143 (The current URI for this page, for reference purposes)
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