IMMUNOLOGICAL CHARACTERIZATION OF CYTOSKELETAL PROTEINS ASSOCIATED WITH THE BASAL BODY, AXONEME AND FLAGELLUM ATTACHMENT ZONE OF TRYPANOSOMA-BRUCEI

Woodward, Robert and Carden, Martin J. and Gull, Keith (1995) IMMUNOLOGICAL CHARACTERIZATION OF CYTOSKELETAL PROTEINS ASSOCIATED WITH THE BASAL BODY, AXONEME AND FLAGELLUM ATTACHMENT ZONE OF TRYPANOSOMA-BRUCEI. Parasitology, 111 . pp. 77-85. ISSN 0031-1820. (The full text of this publication is not available from this repository)

The full text of this publication is not available from this repository. (Contact us about this Publication)

Abstract

The monoclonal antibody BS7, raised to bovine sperm flagellum cytoskeletal antigens in a previous study, is here reported to detect flagellum-associated structures in Trypanosoma brucei and Crithidia fasciculata. Immunoblotting showed that BS7 cross-reacts with several cytoskeletal T. brucei proteins but phosphatase treatment did not diminish this complex immunoblot reactivity. To characterize further the cross-reactive proteins recognized in T. brucei-cytoskeletons by BS7 each was excised from preparative gels and used as an immunogen for antiserum production. Two proteins, with apparent sizes around 43 and 47 kDa, produced antisera shown to be monospecific by immunoblotting total T. brucei flagellum preparations. Each of these detected the basal body-associated immunofluorescence in T. brucei. Identification of the smaller, 43 kDa, component as a basal body-associated product was supported by the behaviour of a second monoclonal antibody, BBA4, which was also shown to detect the T. brucei basal body complex by immunofluorescence and immunoblots the 43 kDa polypeptide. These observations reveal new components of the trypanosome cytoskeleton. Also, they provide a further example of an immunological approach for identification of interesting, rare components of the T. brucei cytoskeleton starting from a complex mixture of proteins.

Item Type: Article
Uncontrolled keywords: TRYPANOSOME; CYTOSKELETON; BASAL BODIES; FLAGELLUM; ANTIBODY
Subjects: Q Science > Q Science (General)
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 15 May 2009 19:38
Last Modified: 14 May 2014 09:40
Resource URI: http://kar.kent.ac.uk/id/eprint/19135 (The current URI for this page, for reference purposes)
  • Depositors only (login required):