[H-3] Nemonapride And [H-3] Spiperone Label Equivalent Numbers Of D-2 And D-3 Dopamine-Receptors In A Range Of Tissues And Under Different Conditions

Vile, J.M. and Dsouza, U.M. and Strange, P.G. (1995) [H-3] Nemonapride And [H-3] Spiperone Label Equivalent Numbers Of D-2 And D-3 Dopamine-Receptors In A Range Of Tissues And Under Different Conditions. Documentation. Lippincott-Raven Publ, Philadelphia, USA (The full text of this publication is not available from this repository)

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Abstract

[H-3]Nemonapride and [H-3]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [H-3] nemonapride and [H-3]spiperone give markedly different B-max values for preparations of D-2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D-2(short), D-2(long), and D-3] in different buffers. B-max values derived using either radioligand differ by an average of <20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [H-3]spiperone and [H-3]nemonapride do not differentiate between different forms or populations of D-2-like receptors.

Item Type: Monograph (Documentation)
Additional information: Journal of Neurochemistry Volume 64 Issue 2, Pages 940 - 943
Uncontrolled keywords: D2 dopamine receptor • D3 dopamine receptor • Antagonist binding
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 25 Oct 2009 18:55
Last Modified: 25 Oct 2009 18:55
Resource URI: http://kar.kent.ac.uk/id/eprint/19109 (The current URI for this page, for reference purposes)
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