Cross, S.J. and Ciruela, A. and Poomputsa, K. and Romaniec, M.P.M. and Freedman, R.B. (1996) Thermostable chaperonin from Clostridium thermocellum. Biochemical Journal, 316 . pp. 615-622. ISSN 0264-6021.
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Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity chromatography and the Cpn10 protein was purified by gel-filtration, ion-exchange and hydrophobic interaction chromatographies. The identities of the proteins were confirmed by N-terminal sequence analysis and antigenic cross-reactivity. The Cpn60 homologue is a weak, thermostable ATPase (t(1/2) at 70 degrees C more than 90 min) with optimum activity (k(cat) 0.07 s(-1)) between 60 degrees C and 70 degrees C. The ATPase activity of the authentic Cpn60 was inhibited by Escherichia coli GroES. The catalytic properties of a recombinant C. thermocellum Cpn60 purified from a GST-Cpn60 fusion protein expressed in E. coli [Ciruela (1995) Ph.D. Thesis, University of Kent] were identical with those of the authentic C. thermocellum Cpn60. Gel-filtration studies show that at room temperature the Cpn60 migrates mainly as a heptamer. Electron microscopy confirms the presence of complexes showing 7-fold rotational symmetry and also reveals a small number of particles that seem to be tetradecamers with a similar structure to E. coli GroEL complexes.
|Additional information:||Part: Part 2|
Q Science > QP Physiology (Living systems) > QP517 Biochemistry
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||M.A. Ziai|
|Date Deposited:||14 May 2009 19:44|
|Last Modified:||16 May 2009 05:36|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18900 (The current URI for this page, for reference purposes)|
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