Castro, P.M.L. and Ison, A.P. and Hayter, P.M. and Bull, A.T. (1996) CHO cell growth and recombinant interferon-gamma production: Effects of BSA, Pluronic and lipids. Cytotechnology, 19 (1). pp. 27-36. ISSN 0920-9069.
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The role of bovine serum albumin in mammalian cell cultures and the possibility of its substitution by other components in a serum-free medium has been investigated. In this study, BSA was shown to be important for growth and product formation in CHO cells expressing recombinant human interferon-gamma. There were indications that its stimulating growth effect was dependent on the source of BSA used and probably was related to the purification procedure used for the production of the desired albumin fraction. Cell growth did not occur in the absence of BSA but at low concentration (1 mg ml(-1)) it was stimulated by the addition of a combination of a commercial lipid mixture plus Pluronic F68. However, under the latter conditions IFN-gamma production was adversely effected. The importance of individual lipid components was investigated using a statistical approach based on a Plackett-Burman design. Linoleic acid was identified as a positive variable for cell growth while cholesterol was identified as a negative variable for both cell growth and IFN-gamma production. When a combination of linoleic acid plus Pluronic F68 was included in the formulation of low BSA medium, cell growth was similar to that at high BSA concentration (5 mg ml(-1)) but the IFN-gamma concentration was significantly reduced (ca. 45%).
|Uncontrolled keywords:||BSA; CHO cells; interferon-gamma; linoleic acid; lipids; Pluronic F68|
Q Science > QR Microbiology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||M.A. Ziai|
|Date Deposited:||15 May 2009 11:33|
|Last Modified:||15 May 2009 11:33|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18866 (The current URI for this page, for reference purposes)|
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