Baillie, G.S. and Hitchcock, C.A. and Burnet, F.R. (1996) Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure. Journal of Medical and Veterinary Mycology, 34 (5). pp. 341-347. ISSN 0268-1218.
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Microsomal fractions were isolated from Aspergillus fumigatus cultures that had been treated with dimethylsulphoxide (DMSO), ethanol, benzopyrene, phenobarbital or naphthalene. All these xenobiotics increased microsomal cytochrome P-450 content. Cytochrome P-450 reductase activity remained unaffected and no alteration in the microsomal protein profile was detectable by SDS PAGE. Benzopyrene, dimethylaniline, hexobarbitol and p-nitroanisole were metabolised by A. fumigatus microsomes, while aniline, ethoxyresorufin and pentoxyresorufin were not. No xenobiotic elicited a more specific oxidation of any of the substrates. A. fumigatus microsomes also catalyzed the cell-free biosynthesis of ergosterol and quantitative analysis of assay metabolites showed that exposure to ethanol, benzopyrene or phenobarbitone in vivo resulted in enhanced ergosterol biosynthesis in vitro.
|Uncontrolled keywords:||Aspergillus; cytochrome P-450; ergosterol biosynthesis; xenobiotics|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||M.A. Ziai|
|Date Deposited:||05 Oct 2009 07:21|
|Last Modified:||05 Oct 2009 07:21|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18793 (The current URI for this page, for reference purposes)|
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