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Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure

Baillie, G.S., Hitchcock, C.A., Burnet, Frank R. (1996) Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure. Journal of Medical and Veterinary Mycology, 34 (5). pp. 341-347. ISSN 0268-1218. (doi:10.1080/02681219680000581) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18793)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1080/02681219680000581

Abstract

Microsomal fractions were isolated from Aspergillus fumigatus cultures that had been treated with dimethylsulphoxide (DMSO), ethanol, benzopyrene, phenobarbital or naphthalene. All these xenobiotics increased microsomal cytochrome P-450 content. Cytochrome P-450 reductase activity remained unaffected and no alteration in the microsomal protein profile was detectable by SDS PAGE. Benzopyrene, dimethylaniline, hexobarbitol and p-nitroanisole were metabolised by A. fumigatus microsomes, while aniline, ethoxyresorufin and pentoxyresorufin were not. No xenobiotic elicited a more specific oxidation of any of the substrates. A. fumigatus microsomes also catalyzed the cell-free biosynthesis of ergosterol and quantitative analysis of assay metabolites showed that exposure to ethanol, benzopyrene or phenobarbitone in vivo resulted in enhanced ergosterol biosynthesis in vitro.

Item Type: Article
DOI/Identification number: 10.1080/02681219680000581
Uncontrolled keywords: Aspergillus; cytochrome P-450; ergosterol biosynthesis; xenobiotics
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: M.A. Ziai
Date Deposited: 05 Oct 2009 07:21 UTC
Last Modified: 16 Nov 2021 09:57 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18793 (The current URI for this page, for reference purposes)

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