Paternoster, M.P. and Burns, R.G. (1996) A novel medium for the oosporogenesis of Aphanomyces cochlioides. Mycological Research, 100 . pp. 936-938. ISSN 0953-7562.
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Reliable production of infective spores is an essential requirement in the screening of putative biological control agents. We report the development of a novel and simple medium for the production of viable oospores of Aphanomyces cochlioides, one of the principal causes of damping-off of sugar beet. The semi-solid medium consists of 5 g l(-1) corn meal agar and 1 g l(-1) mycological peptone and supports the development of 2.25 x 10(5) oospores cm(-2) in 5 d. In a plant bioassay for post-emergence damping-off, the oospores had a consistent pathogenic effect with 50+/-5.7% damping-off induced by 170 oospores g(-1) soil (-15 kPa matric potential) after 20 d. Higher numbers (1700 g(-1) soil) produced 88.8+/-9.1% disease in emerged plants after 20 d.
Q Science > QH Natural history > QH301 Biology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||F.D. Zabet|
|Date Deposited:||03 Jun 2009 16:26|
|Last Modified:||03 Jun 2009 16:26|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18718 (The current URI for this page, for reference purposes)|
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