A novel medium for the oosporogenesis of Aphanomyces cochlioides

Paternoster, M.P. and Burns, Richard G. (1996) A novel medium for the oosporogenesis of Aphanomyces cochlioides. Mycological Research, 100 . pp. 936-938. ISSN 0953-7562. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/S0953-7562(96)80044-X

Abstract

Reliable production of infective spores is an essential requirement in the screening of putative biological control agents. We report the development of a novel and simple medium for the production of viable oospores of Aphanomyces cochlioides, one of the principal causes of damping-off of sugar beet. The semi-solid medium consists of 5 g l(-1) corn meal agar and 1 g l(-1) mycological peptone and supports the development of 2.25 x 10(5) oospores cm(-2) in 5 d. In a plant bioassay for post-emergence damping-off, the oospores had a consistent pathogenic effect with 50+/-5.7% damping-off induced by 170 oospores g(-1) soil (-15 kPa matric potential) after 20 d. Higher numbers (1700 g(-1) soil) produced 88.8+/-9.1% disease in emerged plants after 20 d.

Item Type: Article
Subjects: Q Science
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: F.D. Zabet
Date Deposited: 03 Jun 2009 16:26
Last Modified: 13 May 2014 13:21
Resource URI: http://kar.kent.ac.uk/id/eprint/18718 (The current URI for this page, for reference purposes)
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