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Evaluation of poly (glycerol-adipate) nanoparticle uptake in an In Vitro 3-D brain tumor co-culture model

Meng, Wei, Kallinteri, Paraskevi, Walker, D.A., Parker, T.L., Garnett, Martin C. (2007) Evaluation of poly (glycerol-adipate) nanoparticle uptake in an In Vitro 3-D brain tumor co-culture model. Experimental Biology and Medicine, 232 (8). pp. 1100-1108. ISSN 1535-3702. (doi:10.3181/0612-RM-301) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18622)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.3181/0612-RM-301

Abstract

Despite the inherent problems associated with in vivo animal models of tumor growth and metastases, many of the current in vitro brain tumor models also do notaccurately mimic tumor-host brain interactions. Therefore, there is a need to develop such co-culture models to study tumor biology and, importantly, the efficacy of drug delivery systems targeting the brain. So far, few investigations of this nature have been published. In this paper we describe the development of a new model system and its application to drug delivery assessment. For our new model, a co-culture of DAOY cell brain tumor aggregates and organo-typic brain slices was developed. Initially, the DAOY aggregates attached to cerebellum slices and invaded as a unit. Single cells in the periphery of the aggregate detached from the DAOY aggregates and gradually replaced normal brain cells. This invasive behavior of DAOY cells toward organotypic cerebellum slices shows a similar pattern to that seen in vivo. A er validation of the co-culture model using transmission electron microscopy, nanoparticle (NP) uptake was then evaluated. Confocal micrographs illustrated that DAOY cells in this co-culture model took up most of the NPs, but few NPs were distributed into brain cells. This finding corresponded with results of NP uptake in DAOY and brain aggregates reported elsewhere.

Item Type: Article
DOI/Identification number: 10.3181/0612-RM-301
Additional information: 204TJ Times Cited:2 Cited References Count:27
Uncontrolled keywords: biodegradable nanoparticles medulloblastoma organotypic culture glioma invasion cell-migration system medulloblastomas cultures cancer tissue
Subjects: R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Paraskevi Kallinteri
Date Deposited: 29 Jun 2011 09:05 UTC
Last Modified: 16 Nov 2021 09:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18622 (The current URI for this page, for reference purposes)

University of Kent Author Information

Kallinteri, Paraskevi.

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