Meng, W. and Kallinteri, P. and Walker, D.A. and Parker, T.L. and Garnett, M.C. (2007) Evaluation of poly (glycerol-adipate) nanoparticle uptake in an In Vitro 3-D brain tumor co-culture model. Experimental Biology and Medicine, 232 (8). pp. 1100-1108. ISSN 1535-3702.
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Despite the inherent problems associated with in vivo animal models of tumor growth and metastases, many of the current in vitro brain tumor models also do notaccurately mimic tumor-host brain interactions. Therefore, there is a need to develop such co-culture models to study tumor biology and, importantly, the efficacy of drug delivery systems targeting the brain. So far, few investigations of this nature have been published. In this paper we describe the development of a new model system and its application to drug delivery assessment. For our new model, a co-culture of DAOY cell brain tumor aggregates and organo-typic brain slices was developed. Initially, the DAOY aggregates attached to cerebellum slices and invaded as a unit. Single cells in the periphery of the aggregate detached from the DAOY aggregates and gradually replaced normal brain cells. This invasive behavior of DAOY cells toward organotypic cerebellum slices shows a similar pattern to that seen in vivo. A er validation of the co-culture model using transmission electron microscopy, nanoparticle (NP) uptake was then evaluated. Confocal micrographs illustrated that DAOY cells in this co-culture model took up most of the NPs, but few NPs were distributed into brain cells. This finding corresponded with results of NP uptake in DAOY and brain aggregates reported elsewhere.
|Additional information:||204TJ Times Cited:2 Cited References Count:27|
|Uncontrolled keywords:||biodegradable nanoparticles medulloblastoma organotypic culture glioma invasion cell-migration system medulloblastomas cultures cancer tissue|
|Subjects:||R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)|
|Divisions:||Faculties > Science Technology and Medical Studies > Medway School of Pharmacy|
|Depositing User:||Paraskevi Kallinteri|
|Date Deposited:||29 Jun 2011 09:05|
|Last Modified:||11 Jan 2012 10:48|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18622 (The current URI for this page, for reference purposes)|
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