Uptake and metabolism of novel biodegradable poly(glycerol-adipate) nanoparticles in DAOY monolayer

Meng, Wei and Parker, T.L. and Kallinteri, Paraskevi and Walker, D.A. and Higgins, Sean and Hutcheon, Gillian A. and Garnett, Martin C. (2006) Uptake and metabolism of novel biodegradable poly(glycerol-adipate) nanoparticles in DAOY monolayer. Journal of Controlled Release, 116 (3). pp. 314-321. ISSN 0168-3659. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/j.jconrel.2006.09.014

Abstract

A useful route for the development of antitumour therapies is by creating improved methods for delivering therapeutic agents to tumour cells or subcellular compartments and increasing retention of drugs within target cells. In this study, we have characterized nanoparticle (NP) uptake and metabolism by DAOY cells, a human medulloblastoma cell line. NPs were formed from a novel polymer, poly (glycerol-adipate) (PGA), containing Rhodamine B Isothiocyanate (RBITC) as a fluorescent marker. It was observed that the cellular uptake of NPs depends on the incubation time and the concentration of NPs in the culture medium. The studies of retention and metabolism of NPs within cells indicated that 1) faster degradation of NPs within cells compared with that in cell culture medium in vitro; 2) a small fraction of NPs were recycled back to the outside of cell, whereas most NPs entered endosomes and lysosomes; and 3) recycled NPs were re-taken up in the following 2 h incubation time. These studies thus suggested that PGA NPs could be used for localising therapeutic agents into cells, and could provide prolonged drug effects because of their long sustained release in physiological conditions and their rapid release when taken up into cells.

Item Type: Article
Additional information: 125UA Times Cited:6 Cited References Count:22
Uncontrolled keywords: parenteral delivery biodegradable polymer poly (glycerol-adipate) nanoparticle uptake nanoparticle degradation high-dose carboplatin drug-delivery brain-tumors medulloblastoma release permeability endocytosis Parenteral delivery; Biodegradable polymer; Poly (glycerol-adipate); Nanoparticle uptake; Nanoparticle degradation
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Paraskevi Kallinteri
Date Deposited: 02 Jun 2009 07:50
Last Modified: 25 Apr 2014 14:14
Resource URI: http://kar.kent.ac.uk/id/eprint/18621 (The current URI for this page, for reference purposes)
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