Investigation of the role of conserved serine residues in the long form of the rat D-2 dopamine receptor using site-directed mutagenesis

Woodward, Robert and Coley, Clare and Daniell, Sarah J. and Naylor, Louise H. and Strange, Philip G. (1996) Investigation of the role of conserved serine residues in the long form of the rat D-2 dopamine receptor using site-directed mutagenesis. Journal of Neurochemistry, 66 (1). pp. 394-402. ISSN 0022-3042. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1046/j.1471-4159.1996.6601039...

Abstract

Three serine residues (Ser(193), Ser(194), Ser(197)) in the fifth transmembrane-spanning region of the D-2 dopamine receptor have been mutated separately to alanine and the effects of the mutations determined in ligand-binding experiments with [H-3]spiperone. For many antagonists the mutations had little effect, showing that the overall conformation of the mutant receptors was similar to that of the native, although there were effects on the binding of certain antagonists. The effect of the mutations on agonist binding to the free receptor (uncoupled from G proteins) was determined in the presence of GTP (100 mu M). This showed that there was no single mode of binding of catecholamine agonists to the receptor and that ail three serine residues can participate in the binding of some agonists, possibly through hydrogen bonds to the catechol hydroxyl groups. Coupling of the mutant receptors to G proteins was assessed from agonist-binding curves in the absence of GTP, when higher and lower affinity agonist-binding sites were seen. Receptor/G protein coupling was generally unaffected by the Ala(193) and Ala(194) mutations, but the Ala(197) mutation eliminated receptor/G protein coupling for some agonists. These data show that the interactions of agonists with the free and coupled forms of the receptor are different.

Item Type: Article
Uncontrolled keywords: D-2 dopamine receptor; mutagenesis; agonist; antagonist; serine; hydrogen bond
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 28 Apr 2009 17:26
Last Modified: 30 Jun 2014 15:10
Resource URI: http://kar.kent.ac.uk/id/eprint/18592 (The current URI for this page, for reference purposes)
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