Ciruela, A. and Cross, S. and Freedman, R.B. and Hazlewood, G.P. (1997) Sequence and transcriptional analysis of groES and groEL genes from the thermophilic bacterium Clostridium thermocellum. Gene, 186 (1). pp. 143-147. ISSN 0378-1119.
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The groESL operon from Clostridium thermocellum (Ct) has been isolated and sequenced, revealing two ORFs of 285 and 1626 nt, separated by 48 nt. The first ORF encoded a 94-aa 10.6-kDa GroES homologue; the second encoded a 541-aa polypeptide of 57.6 kDa, that exhibited 61% and 77% sequence identity with GroEL from Escherichia coli (Ec) and Clostridium acetobutylicum (Ca), respectively. A putative tsp, preceded by -10 and -35 consensus promoters, was identified upstream of groES. This was followed by an inverted repeat observed previously in bacterial heat shock genes. A 15-nt palindrome characteristic of a Rbo-independent transcription terminator, was located downstream of groEL. The first nt of the groES translational start codon was preceded (7 nt) by a putative RBS (AGGAGG); a second RBS sequence was located 8 nt upstream of the groEL start. production of GroE homologues by Ct was constitutive, but was enhanced significantly during a temperature upshift from 60 degrees C to 70 degrees C. The Ct GroEL, expressed in Ec as a fusion protein with GST, was purified, free of contaminating Ec GroEL.
|Depositing User:||T. Nasir|
|Date Deposited:||25 Oct 2009 11:52|
|Last Modified:||25 Oct 2009 11:52|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18352 (The current URI for this page, for reference purposes)|
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