Welsh, G.I. and Patel, J.C. and Proud, C.G. (1997) Peptide substrates suitable for assaying glycogen synthase kinase-3 in crude cell extracts. Analytical Biochemistry, 244 (1). pp. 16-21. ISSN 0003-2697.
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In this study we describe the characterization and use of new peptide substrates for assaying glycogen synthase kinase-3 (GSK-3) which are based on the sequence around the single GSK-3 phosphorylation site in the translation factor eIF2B. The new peptides offer important advantages over previous substrates, which were based on the sequence around the multiple GSK-3 phosphorylation sites in glycogen synthase (GS), for the assay of GSK-3 in cell extracts. In particular, decreases in GSK-3 activity following, e.g., insulin treatment, are partially or completely masked when the GS-based peptides are used but are readily measured using the new, eIF2B-based, peptides. The new peptides, unlike those based on GS, are therefore suitable for the assay of changes in GSK-3 activity in cell extracts without the need for prior immunoprecipitation or ion-exchange chromatography. (C) 1997 Academic Press, Inc.
|Subjects:||Q Science > QD Chemistry|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||T.J. Sango|
|Date Deposited:||29 Apr 2009 13:02|
|Last Modified:||29 Apr 2009 13:02|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/18037 (The current URI for this page, for reference purposes)|
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