Distant cis-active sequences and sialic acid control the expression of fimB in Escherichia coli K-12.

El-Labany, Sammia and Sohanpal, Baljinder K. and Lahooti, Maryam and Akerman, Robert and Blomfield, Ian C. (2003) Distant cis-active sequences and sialic acid control the expression of fimB in Escherichia coli K-12. Molecular Microbiology, 49 (4). pp. 1109-1118. ISSN 0950-382X. (Access to this publication is restricted)

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Abstract

The phase variation of type 1 fimbriation in Escherichia coli is controlled by the inversion of a 314 bp element of DNA, determined by FimB (switching in both directions) or FimE (switching from the ON-to-OFF orientation predominantly), and influenced by auxiliary factors IHF, Lrp and H-NS. The fimB gene is separated from the divergently transcribed yjhATS operon by a large (1.4 kbp) intergenic region of unknown function. Here, we show that fimB expression is regulated by multiple cis-active sequences that lie far upstream (>600 bp) of the transcription start sites for the recombinase gene. Two regions characterized further (regions 1 and 2) show sequence identity, and each coincides with a methylation-protected Dam (5'-GATC) site. Regions 1 and 2 apparently control fimB expression by an antirepression mechanism that involves additional sequences proximal to yjhA. Region 1 encompasses a 27 bp DNA sequence conserved upstream of genes known (nanAT ) or suspected (yjhBC) to be involved in sialic acid metabolism, and we show that FimB expression and recombination are suppressed by N-acetylneuraminic acid. We propose that E. coli recognizes the amino sugars as a harbinger of potential host defence activation, and suppresses the expression of type 1 fimbriae in response.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 19 Dec 2007 17:44
Last Modified: 06 May 2014 15:42
Resource URI: http://kar.kent.ac.uk/id/eprint/18 (The current URI for this page, for reference purposes)
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