Tett, V.A and Willetts, A.J. and Lappin-Scott, H.M. (1997) Biodegradation of the chlorophenoxy herbicide (R)-(+)-mecoprop by Alcaligenes denitrificans. Biodegradation, 8 (1). pp. 43-52. ISSN 0923-9820.
|The full text of this publication is not available from this repository. (Contact us about this Publication)|
An Alcaligenes denitrificans strain capable of utilizing the herbicide (R)-(+)-2(2-methyl-4-chlorophenoxy)propionic acid (mecoprop) as a sole carbon source was isolated from soil and cultured in liquid medium. Crude cell extracts of the bacterium were utilized in spectrophotometric assays to elucidate a biochemical pathway for degradation of mecoprop. Results indicated a reaction sequence analogous to the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D). GC-MS analysis provided direct evidence for the biotransformation of mecoprop to the transient metabolite 4-chloro-2-methylphenol (MCP). No NADPH-dependent activity was observed during this reaction. Pyruvate was verified as the second product derived from the aliphatic side chain of mecoprop. MCP was subsequently transformed to a substituted catechol by an NADPH-dependent monooxygenase. When grown on mecoprop, A. denitrificans was adapted to oxidize catechol and its 3- and 3-methylated derivatives indicating the broad substrate specificity of catechol dioxygenase. The microorganism was demonstrated to adopt the ortho mechanism of aromatic cleavage which resulted in the formation of 2-methyl-3-carboxymethylene but-2-en-4-olide, a reaction intermediate of the beta-ketoadipate pathway.
|Uncontrolled keywords:||Alcaligenes denitrificans; biodegradation; chloroaromatic; mecoprop (R)-(+)-2(2-methyl-4-chlorophenoxy)propio|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||T.J. Sango|
|Date Deposited:||23 Sep 2009 12:13|
|Last Modified:||01 Aug 2012 08:10|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/17992 (The current URI for this page, for reference purposes)|
- Depositors only (login required):