Maldonado, L.A. and Stach, J.E. and Ward, A.C. and Bull, A.T. and Goodfellow, M. (2008) Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods. Antonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology, 94 (2). pp. 289-298. ISSN 0003-6072 .
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Large numbers of strains assigned to the genus Micromonospora on the basis of typical colonial and pigmentation features were isolated from diverse aquatic sediments using a standard selective isolation procedure. Two hundred and six isolates and eight representatives of the genus Micromonospora were assigned to 24 multimembered groups based on a numerical analysis of banding patterns generated using BOX and ERIC primers. Representatives of multimembered groups encompassing isolated micromonosporae were the subject of 16S rRNA gene sequencing analyses. Good congruence was found between the molecular fingerprinting and 16S rRNA sequence data indicating that the groups based upon the former are taxonomically meaningful. Nearly all of the isolates that were chosen for the 16S rRNA gene sequencing analyses showed that the ecosystems studied are a rich source of novel micromonosporae. These findings have implications for high throughput screening for novel micromonosporae as BOX and ERIC fingerprinting, which is rapid and reproducible, can be applied as a robust dereplication procedure to indicate which environmental isolates have been cultured previously.
|Additional information:||0003-6072 (Print) Evaluation Studies Journal Article Research Support, Non-U.S. Gov't|
|Uncontrolled keywords:||DNA Fingerprinting/*methods DNA, Bacterial/genetics DNA, Ribosomal/genetics Geologic Sediments/*microbiology Micromonospora/*classification/genetics/*isolation & purification Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA *Water Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||Sue Davies|
|Date Deposited:||21 Apr 2009 10:15|
|Last Modified:||12 May 2009 12:48|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/17851 (The current URI for this page, for reference purposes)|
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