Nerve and epidermal growth factor induce protein synthesis and eIF2B activation in PC12 cells

Kleijn, Miranda and Welsh, Gavin I. and Scheper, Gert C. and Voorma, Harry O. and Proud, Christopher G. and Thomas, Adri A.M. (1998) Nerve and epidermal growth factor induce protein synthesis and eIF2B activation in PC12 cells. Journal of Biological Chemistry, 273 (10). pp. 5536-5541. ISSN 0021-9258. (The full text of this publication is not available from this repository)

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Abstract

The regulation of protein synthesis and of eukaryotic initiation factor eIF2B was studied in PC12 cells. An increase in protein synthesis was observed after nerve growth factor (NGF) and epidermal growth a factor (EGF) treatment of PC12 cells, and this increase coincided with activation of eIF2B. Growth factor addition in the presence of the phosphatidylinositol-3'-OH kinase inhibitor wortmannin showed that both NGF- and EGF-induced protein synthesis and eIF2B activation were phosphatidylinasitol-3'-OH kinase dependent. The EGF-induced stimulation of protein synthesis and activation of eIF2B was dependent upon FK506-binding protein-rapamycin-associated protein, as shown with the immunosuppressant rapamycin, whereas NGF induction was partially dependent upon FK506-bindinag protein-rapamycin-associated protein. The activities of two kinases that act on eIF2B, glycogen synthase kinase-3 and casein kinase II, were measured to assess their potential roles in the activation of eIF2B in PC12 cells. inactivation of glycogen synthase kinase-3 was seen in response to both NGF and EGF and this coincided with activation of eIF2B. However, inactivation of glycogen synthase kinase-3 was not rapamycin sensitive, in contrast to the activation of eIF2B. This indicates the involvement of another protein kinase or regulatory mechanism in the eIF2B activation. Both growth factors activated casein kinase II. However, the time course of its activation and its insensitivity to wortmannin and rapamycin suggest that casein kinase II does not play a major regulatory role in eIF2B activation under these conditions.

Item Type: Article
Subjects: Q Science
Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M.A. Ziai
Date Deposited: 17 Aug 2009 07:47
Last Modified: 10 Jun 2014 08:34
Resource URI: http://kar.kent.ac.uk/id/eprint/17401 (The current URI for this page, for reference purposes)
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