The b ' domain provides the principal peptide-binding site of protein disulfide isomerase but all domains contribute to binding of misfolded proteins

Klappa, P. and Ruddock, L.W. and Darby, N.J. and Freedman, R.B. (1998) The b ' domain provides the principal peptide-binding site of protein disulfide isomerase but all domains contribute to binding of misfolded proteins. Embo Journal, 17 (4). pp. 927-935. ISSN 0261-4189. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1093/emboj/17.4.927

Abstract

Protein disulfide isomerase (PDI) is a very efficient catalyst of folding of many disulfide-bonded proteins, A great deal is known about the catalytic functions of PDI, while little is known about its substrate binding, We recently demonstrated by cross-linking that PDI binds peptides and misfolded proteins, with high affinity but broad specificity. To characterize the substrate-binding site of PDI, we investigated the interactions of various recombinant fragments of human PDI, expressed in Escherichia coil, with different radiolabelled model peptides, We observed that the b' domain of human PDI is essential and sufficient for the binding of small peptides, In the case of larger peptides, specifically a 28 amino acid fragment derived from bovine pancreatic trypsin inhibitor, or misfolded proteins, the b' domain is essential but not sufficient for efficient binding, indicating that contributions from additional domains are required, Hence we propose that the different domains of PDI all contribute to the binding site, with the b' domain forming the essential core.

Item Type: Article
Uncontrolled keywords: cross-linking; domain; peptide binding; protein disulfide isomerase
Subjects: Q Science
Q Science > QD Chemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M.A. Ziai
Date Deposited: 04 Apr 2009 22:17
Last Modified: 04 Apr 2009 22:17
Resource URI: http://kar.kent.ac.uk/id/eprint/17399 (The current URI for this page, for reference purposes)
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