Eaglestone, S.S. and Cox, B.S. and Tuite, M.F. (1999) Translation termination efficiency can be regulated in Saccharomyces cerevisiae by environmental stress through a prion-mediated mechanism. Embo Journal, 18 (7). pp. 1974-1981. ISSN 0261-4189.
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| Official URL http://dx.doi.org/10.1093/emboj/18.7.1974 |
Abstract
[PSI+] is a protein-based heritable phenotype of the yeast Saccharomyces cerevisiae which reflects the prion-like behaviour of the endogenous Sup35p protein release factor. [PSI+] strains exhibit a marked decrease in translation termination efficiency, which permits decoding of translation termination signals and, presumably, the production of abnormally extended polypeptides. We have examined whether the [PSI+]-induced expression of such an altered proteome might confer some selective growth advantage over [psi(-)] strains. Although otherwise isogenic [PSI+] and [psi(-)] strains show no difference in growth rates under normal laboratory conditions, we demonstrate that [PSI+] strains do exhibit enhanced tolerance to heat and chemical stress, compared with [psi(-)] strains. Moreover, we also show that the prion-like determinant [PSI+] is able to regulate translation termination efficiency in response to environmental stress, since growth in the presence of ethanol results in a transient increase in the efficiency of translation termination and a loss of the [PSI+] phenotype. We present a model to describe the prion-mediated regulation of translation termination efficiency and discuss its implications in relation to the potential physiological role of prions in S. cerevisiae and other fungi.
| Item Type: | Article |
|---|---|
| Uncontrolled keywords: | environmental stress; phenotype; prion; yeast (Saccharomyces cerevisiae) |
| Subjects: | Q Science |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | M. Nasiriavanaki |
| Date Deposited: | 21 Jun 2009 11:00 |
| Last Modified: | 21 Jun 2009 11:00 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/17309 (The current URI for this page, for reference purposes) |
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