Welsh, G.I. and Hall, D.A. and Warnes, A. and Strange, P.G. and Proud, C.G. (1998) Activation of microtubule-associated protein kinase (Erk) and p70 S6 kinase by D-2 dopamine receptors. Journal of Neurochemistry, 70 (5). pp. 2139-2146. ISSN 0022-3042.
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The ability of human and rat D-2(short) and D-2(long) dopamine receptors to activate microtubule-associated protein (MAP) kinase (Erk1/2) and p70 S6 kinase has been investigated in recombinant cells expressing these receptors. In cells expressing the D-2(short) receptor, dopamine activated both enzymes in a transient manner but with very different time courses, with activation of Erk being much quicker. Activation of both enzymes by dopamine was dose-dependent and could be prevented by a range of selective dopamine antagonists. Excellent correlations were observed between the potencies of the antagonists for blocking enzyme activation and their affinities for the D-2 dopamine receptor. Activation of Erk and of p70 S6 kinase via the D-2 dopamine receptors was prevented by pretreatment of the cells with pertussis toxin, indicating the involvement-of G proteins of the G(i) or G(o) family. Inhibitors of phosphatidylinositol 3-kinase (PI 3-kinase) were found to block substantially, but not completely, activation of p70 S6 kinase by dopamine, suggesting the involvement of PI 3-kinase-dependent and -independent signalling pathways in its control by dopamine, p70 S6 kinase activation was completely blocked by rapamycin. In the case of Erk, activation was partially blocked by wortmannin or LY294002, indicating a possible link with PI 3-kinase.
|Uncontrolled keywords:||dopamine; D-2 receptor; microtubule-associated protein kinase; p70 S6 kinase|
|Subjects:||R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Tara Puri|
|Date Deposited:||25 Mar 2009 17:51|
|Last Modified:||25 Mar 2009 17:51|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/17271 (The current URI for this page, for reference purposes)|
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