Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp

Clapp, J.P. and Fitter, A.H. and Young, J.P.W. (1999) Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology, 8 (6). pp. 915-921. ISSN 0962-1083. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1046/j.1365-294x.1999.00642.x

Abstract

Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire, UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceae-specific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales, with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.

Item Type: Article
Uncontrolled keywords: 18S, arbuscular mycorrhiza, diversity, Glomales, rRNA, Scutellospora , SSU, VANS1
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M. Nasiriavanaki
Date Deposited: 25 Jun 2009 07:52
Last Modified: 25 Jun 2009 07:52
Resource URI: http://kar.kent.ac.uk/id/eprint/17217 (The current URI for this page, for reference purposes)
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