Sporulation at minimum specific growth rate in Aspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations

Bushell, M.E. and Bull, A.T. (1999) Sporulation at minimum specific growth rate in Aspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations. Journal of Basic Microbiology, 39 (5-6). pp. 293-298. ISSN 0233-111X. (The full text of this publication is not available from this repository)

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http://dx.doi.org/10.1002/(SICI)1521-4028(199912)3...

Abstract

Ribosomal efficiency (RE) estimates provide a quantitative descriptor of intrinsic growth rate of cell populations using readily-obtainable experimental data. In Aspergillus nidulans chemostat cultures, RE increased linearly with growth rate over the range 25-60% of maximum growth rate (mu(max)) consistent with increasing ribosomal usage with increased growth rate. Above 60%, RE did not in crease significantly, suggesting that all ribosomes were functional at 60% of mu(max), further increases in growth rate, presumably resulting from increased polypeptide chain elongation rate. Extrapolating the linear part of the RE/growth rate curve predicted zero RE at a growth rate of 0.04 h(-1). Chemostat steady state cultures at 0.04 h(-1) contained spores (conidia), apparently undergoing a continuous sporulation/germination cycle. We propose that the RE estimates provide a means of predicting the value of minimum specific growth rate (mu(min)) below which net growth cannot take place.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M. Nasiriavanaki
Date Deposited: 03 Jul 2009 06:57
Last Modified: 03 Jul 2009 06:57
Resource URI: http://kar.kent.ac.uk/id/eprint/17156 (The current URI for this page, for reference purposes)
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